Small nucleic acid technology is a core tool in current life sciences and gene function research, and the demand for experimental samples such as siRNA, DNA, and modified oligonucleotides continues to surge. From high-throughput library screening and cellular/molecular experiments to large-scale experimental raw material preparation, there are stringent requirements for synthesis throughput, modification capability, and purification quality control. Hangzhou Open Gene has established an integrated small nucleic acid R&D service platform spanning synthesis – purification – formulation – quality inspection, with separate Class 100,000 (ISO 8) and Class 10,000 (ISO 7) cleanroom zones, equipped with a full set of professional synthesis, liquid chromatography, and mass spectrometry detection instruments. Serving university laboratories, research institutes, and biotechnology enterprises, it provides stable, efficient, and high-standard customized small nucleic acid synthesis services, offering one-stop solutions for the entire workflow of oligonucleotide experimental sample preparation. I. Class 100,000 Constant-Temperature-and-Humidity Synthesis Laboratory — High-Throughput, Flexible Multi-Scale Synthesis The synthesis laboratory operates in a Class 100,000 constant-temperature-and-humidity cleanroom environment, with a dual-track production capacity covering both batch screening and large-scale sample preparation: ✅ High-throughput capacity: 800–1,200 siRNAs can be synthesized per day, rapidly completing large-scale gene library screening; ✅ Full coverage of synthesis scales: a single synthesis run spans 10 μg–10 g, accommodating both micro-scale cellular experiments and large-batch animal experiment feedstocks. A matrix of multiple synthesizer models adapts to diverse research needs. 1. 192-channel synthesizer: Equipped with 20 modified-monomer sites, synthesizing 192 strands in parallel per run; single-base synthesis cycle of 8–12 min; synthesis scale 1–1000 nmol, suitable for routine high-throughput screening; supports custom synthesis with various modified monomers such as DNA, RNA, 2'-OMe, and 2'-F. 2. 48-channel synthesizer: 20 modified-monomer positions, synthesizing 48 oligonucleotides per run, with fast synthesis speed, suitable for small-to-medium batch sequence optimization and single-gene targeting studies. 3. 384-channel synthesizer: Equipped with 24 modified-monomer sites, capable of synchronously synthesizing 384 strands per run, ultra-high throughput, suitable for genome-wide high-throughput screening projects. 4. AKTA OP100 synthesizer: 12 modified-monomer sites, single-strand synthesis scale of 10–500 μmol; single-base cycle of 30–45 min, dedicated to large-batch experimental oligonucleotide preparation, meeting the high-volume demands of in vivo animal experiments. The entire equipment series supports customization of various chemically modified backbones; modified nucleic acids such as phosphorothioate, 2'-methyl, and 2'-fluoro can be synthesized on demand, enhancing the in vitro stability of nucleic acids and adapting to diverse experimental scenarios such as transfection and in vivo delivery. II. Class 10,000 Fully Automated Purification Platform — Batch Purification with Stable Delivery of High-Purity Nucleic Acid Samples A dedicated Class 10,000 purification laboratory can synchronously purify 800–1,200 siRNAs per day. The fully automated purification system features automatic sample injection and automatic fraction collection, reducing contamination and errors caused by manual operation: Core purification equipment 1. 100 mL liquid purification instrument: binary pump design, maximum flow rate of 100 mL/min, paired with a 50 mm DAC purification column, meeting the refined purification of large-batch nucleic acid samples. 2. Waters 2795 liquid purification instrument: supports 96-well plate automatic injection and collection, quaternary gradient pump, maximum flow rate of 10 mL/min, the preferred choice for rapid purification of high-throughput screening samples. Purified nucleic acids meet the different experimental standards of cell transfection, in vivo animal experiments, and molecular mechanism studies; short-fragment impurities and unreacted monomers are removed, ensuring experimental reproducibility. III. Supporting Lyophilization Equipment — Stable and Easy-to-Store Products The platform is equipped with professional vacuum freeze-drying equipment, and can directly provide lyophilization (freeze-drying) services upon completion of purification: one-stop desalting, concentration, and lyophilization are completed, with no organic solvent residue in the final product and strict control of RNase and DNase contamination; the lyophilized powdered form is stable, with a longer shelf life under low-temperature storage, facilitating aliquoting, transportation, and use in various in vitro and in vivo experiments, eliminating the need for clients to coordinate multiple outsourcing partners. IV. Full Set of Professional Quality Inspection Equipment — Batch-by-Batch Testing with Reliable and Traceable Experimental Data An independent quality inspection area enables multi-dimensional quality control via LC-MS, liquid chromatography, and moisture detection. A complete test report is provided for every batch of nucleic acids, supporting paper data and project experiments: 1. Thermo LTQ Velos high-resolution LC-MS: 96-well plate automatic injection, capable of resolving nucleic acid molecules up to 60 kDa, precisely verifying nucleic acid molecular weight and modification structure, and confirming sequence integrity. 2. Thermo LCQ LC-MS: suitable for qualitative detection of oligonucleotides at conventional molecular weights, completing high-throughput sample mass spectrometry identification in batches. 3. Agilent 1100 liquid chromatography analyzer: 100-well automatic injection, high-performance liquid chromatography precisely detecting nucleic acid purity and impurity content, quantifying missing-fragment impurities. 4. KLS-3300 micro moisture meter: real-time detection of moisture content in synthesis monomers, activating reagents, and solvents, reducing synthesis side reactions at the source and ensuring modification and coupling synthesis efficiency. From raw material warehousing, the synthesis process, purified products, to lyophilized final products, testing records are retained throughout the entire process; every batch of samples is traceable, ensuring rigorous and reliable experimental data. V. Core Advantages of Open Gene's Small Nucleic Acid Platform 1. Fully integrated one-stop service: nucleic acid synthesis, purification, lyophilization, and quality inspection are delivered in one place, eliminating the need for multiple outsourcing coordinations, shortening sample delivery cycles, and reducing communication costs. 2. Flexible and adaptive production scales: covers micro-scale nmol-level screening, μmol-level optimization, and gram-scale large-batch preparation, adapting to the full-stage research needs of project preliminary screening, molecular validation, and animal experiments. 3. Rich modification synthesis capability: mature support for various common modifications such as 2'-OMe, 2'-F, and phosphorothioate backbones; targeted modified nucleic acids can be customized to adapt to various delivery system studies. 4. Strict contamination control via clean zoning: Class 100,000 synthesis zone and Class 10,000 purification zone are managed separately, with a nuclease-free process throughout, ensuring stable and reproducible experimental results. VI. Applicable Service Scenarios 🔬 Universities and research institutes: RNA interference mechanism research, high-throughput siRNA library screening, miRNA/ASO functional validation, gene knockdown experiments 🧪 Biotechnology enterprises: target sequence screening, modified nucleic acid development, large-batch experimental nucleic acid raw material preparation, molecular pharmacology experiment sample supply Built on R&D, centered on service, and supported by quality — Hangzhou Open Gene is dedicated to building a professional nucleic acid synthesis R&D service platform. If you are conducting related scientific research experiments on siRNA, modified oligonucleotides, DNA, etc., and need high-throughput synthesis or large-batch customized nucleic acid samples, please leave a message for consultation to obtain a dedicated synthesis plan and quotation!
Oligonucleotide Synthesis Platform
