Principles
Surface Plasmon Resonance (SPR) is a real-time, label-free optical biosensing technology that monitors molecular binding and dissociation processes in real time by detecting refractive index changes near a metal surface.
Core Parameters
- ka (association rate constant): describes how fast molecules bind
- kd (dissociation rate constant): describes how fast molecules dissociate
- KD (equilibrium dissociation constant): kd/ka, measures affinity strength
- RU (Response Unit): reflects surface binding amount in real time
Advantages
- Real-time kinetic monitoring without labeling
- Low sample consumption (µL level)
- Simultaneous acquisition of kinetic and thermodynamic information
- Suitable for protein-small molecule, protein-protein, protein-nucleic acid interactions