Overview
Combining CRISPR-Cas9 gene editing technology with functional phenotypic analysis provides genetic-level causal evidence for drug target functional validation. By knocking out candidate target genes and observing phenotypic changes, we confirm the functional role of targets in disease-related pathways.
Services
- sgRNA design & optimization: Design highly specific sgRNAs for target genes with off-target risk assessment
- Knockout cell line construction: Transfection/infection, single clone screening, genotyping
- Functional phenotype analysis: Multi-dimensional phenotyping including proliferation, apoptosis, migration, signaling pathways
- Rescue experiments: Express drug-resistant mutants to confirm target specificity
Technology Platform
- Supports multiple editing modes: CRISPR/Cas9, CRISPRi, CRISPRa
- Covers tumor cell lines, primary cells, and other models
- Combined validation with Western Blot, qPCR, flow cytometry, etc.