Overview
Site-directed mutagenesis is used to modify key amino acid residues of target proteins, studying the impact of mutations on protein function, stability, and drug binding to precisely elucidate the structural basis of drug action.
Services
- Mutation design & construction: Design point mutations, deletions, insertions based on structural information
- Expression & purification: Recombinant expression system selection and optimization, high-purity protein preparation
- Biochemical characterization: Enzyme activity assay, thermal stability (DSF/Tm), secondary/tertiary structure analysis
- Drug binding analysis: SPR/MST/ITC measurement of affinity differences between wild-type and mutant
Typical Applications
- Confirm key amino acid residues for drug binding
- Elucidate molecular mechanisms of resistance mutations
- Guide structural optimization direction for lead compounds