Design Services
Our oligonucleotide design service integrates multiple algorithmic tools and empirical rules to ensure sequences are both efficient and safe:
- siRNA design: Comprehensive algorithm based on thermodynamic parameters, seed region scores, off-target prediction
- ASO design: Considers GC content, self-dimerization, SNP sites, and other factors
- Gapmer optimization: Optimal ratio of DNA gap length to modified wing length
- Multi-sequence alignment: Cross-species conservation analysis and species-specific design
Screening Services
- In vitro screening: qPCR knockdown efficiency primary screen (96/384-well HTS)
- Cellular functional screening: Phenotype readouts (viability, apoptosis, reporter genes)
- Off-target screening: Transcriptome sequencing (RNA-seq) for comprehensive off-target assessment
- In vivo screening: Efficacy and PK/PD evaluation in organoid/animal models