Overview
Small interfering RNA (siRNA) are double-stranded RNA molecules approximately 19–23 bp in length that induce specific degradation of target mRNAs through the RNA interference (RNAi) pathway. We provide high-quality siRNA design and synthesis services.
Product Types
- Standard siRNA: 19–21 bp duplexes with 3' dTdT overhangs
- Modified siRNA: 2'-OMe/2'-F/PS modifications for enhanced stability
- shRNA expression vectors: Plasmid/lentiviral vector formats
- Dicer-substrate siRNA: 25–27 bp long substrate siRNAs
Quality Control
- MALDI-TOF or ESI-MS molecular weight confirmation
- Ion-exchange HPLC (IEX-HPLC) or UHPLC purity analysis
- Duplex annealing efficiency verification (PAGE electrophoresis)
- Endotoxin testing (< 0.1 EU/mg)